Several agents are used for cross linking in histology:
1. Formaldehyde: A widely used fixative that forms cross links by reacting with amino groups in proteins. 2. Glutaraldehyde: A more potent fixative that reacts with multiple functional groups, providing stronger cross links. 3. Paraformaldehyde: A polymerized form of formaldehyde often used in electron microscopy. 4. Ethanol and Methanol: Alcohols that precipitate proteins and can also induce cross linking to some extent.
