Perfusion can be technically challenging and requires careful control of several parameters: - Flow Rate: The rate at which the fixative is introduced can affect tissue preservation. Too fast, and it can cause tissue damage; too slow, and it may not penetrate effectively. - Pressure: Maintaining appropriate pressure is crucial to ensure that the fixative reaches all parts of the tissue. - Temperature: The temperature of the fixative solution can influence the fixation process. Generally, room temperature is used, but some protocols may require cold solutions.
