Perfusion typically involves several steps: 1. Anesthesia: The animal is anesthetized to minimize suffering. 2. Cannulation: A catheter is inserted into the vascular system, usually through the heart or a major blood vessel. 3. Flushing: The vascular system is flushed with a saline solution to clear blood and prevent clotting. 4. Fixative Introduction: A fixative solution, such as formaldehyde or glutaraldehyde, is then introduced to preserve the tissue.
