Despite its advantages, cell sorting also presents several challenges:
Cell Viability: Some sorting methods can be harsh on cells, leading to reduced viability and functionality. Purity: Achieving high-purity cell populations can be difficult, especially when dealing with rare cell types. Cost and Complexity: Advanced cell sorting techniques like FACS can be expensive and require specialized equipment and expertise. Throughput: Sorting large numbers of cells can be time-consuming, especially with methods that have lower throughput.
