What are the Challenges in Using Oligonucleotide Probes?
Despite their advantages, there are also challenges associated with the use of oligonucleotide probes:
- Probe Design: Designing probes that are highly specific and efficient can be complex and time-consuming. - Hybridization Conditions: The conditions for hybridization must be carefully optimized to ensure specific binding without background noise. - Tissue Preservation: The quality of tissue preservation can affect the efficiency and accuracy of probe hybridization.
