What are the Common Protocols for Using Hematoxylin?
There are several staining protocols involving hematoxylin, with the most widely used being the Hematoxylin and Eosin (H&E) stain. In an H&E stain, hematoxylin stains the nuclei blue, while eosin stains the cytoplasm and extracellular matrix in varying shades of pink and red. Other protocols include the Harris hematoxylin, Mayer's hematoxylin, and Gill's hematoxylin, each with slight variations in composition and staining characteristics.