What Are the Challenges in Studying Biofilms Using Histology?
Studying biofilms using histology presents several challenges: 1. Sample Preparation: Biofilm samples can be difficult to fix, section, and stain due to their complex and heterogeneous nature. 2. Preservation of Structure: Maintaining the integrity of the biofilm's three-dimensional structure during preparation can be challenging. 3. Quantification: Quantifying biofilm components and deciphering the spatial distribution of different microorganisms within the biofilm are complex tasks.
