What are the Challenges in Detecting Target Molecules?
Detecting target molecules can be challenging due to:
Non-specific binding: Antibodies or probes may bind to non-target molecules, leading to false positives. Tissue preservation: The process of fixation and embedding can alter the structure of target molecules, affecting detection. Signal amplification: In cases where the target molecule is present in low quantities, amplifying the signal without increasing noise can be difficult.
