What are the Challenges in Achieving High Specificity and Sensitivity?
Achieving high specificity and sensitivity can be challenging due to factors such as the complexity of biological tissues, the presence of similar proteins or cells, and technical limitations of the testing methods. For example, in immunohistochemistry, cross-reactivity of antibodies with non-target proteins can reduce specificity. Similarly, suboptimal tissue preparation or staining techniques can affect sensitivity. Continuous advancements in technology and rigorous validation of diagnostic tests are essential to overcome these challenges.
