Despite its potential, 3D reconstruction in histology faces several challenges:
Sectioning Artifacts: Physical distortions or damages during tissue sectioning can complicate image alignment and reconstruction. Image Registration: Accurate alignment of 2D images is crucial but can be difficult due to variability in tissue slices. Computational Resources: The process requires significant computational power and storage, especially for high-resolution images. Segmentation: Automated segmentation algorithms may struggle with complex structures, necessitating manual intervention.
