The process of 3D reconstruction typically involves several steps:
Sample Preparation: Biological tissues are fixed, embedded, and sectioned into thin slices using a microtome. Image Acquisition: Each histological section is stained and imaged using various microscopy techniques, such as light microscopy, confocal microscopy, or electron microscopy. Image Alignment: The 2D images are aligned and registered to ensure accurate reconstruction. This step can be challenging due to distortions and differences between sections. Segmentation: Specific structures within the images are identified and delineated, often using advanced image processing algorithms or manual annotation. 3D Reconstruction: The segmented images are stacked and rendered into a 3D model using specialized software.
