Despite their potential, iPSCs come with several challenges:
Genomic Instability: The reprogramming process can introduce genetic mutations, which may affect the functionality and safety of iPSCs. Differentiation Efficiency: Achieving efficient and consistent differentiation of iPSCs into specific cell types remains a significant hurdle. Tumorigenicity: The use of oncogenes like c-Myc in the reprogramming process raises concerns about the potential for iPSCs to form tumors. Ethical Issues: While iPSCs bypass many ethical issues associated with embryonic stem cells, there are still ethical considerations regarding their use in research and therapy.
