Multiplexed tissue imaging typically involves the sequential or simultaneous staining of tissue sections with multiple antibodies, each conjugated to a different fluorophore or mass tag. The stained sections are then analyzed using advanced imaging systems, such as confocal microscopy, multiphoton microscopy, or mass spectrometry-based imaging. Image analysis software is used to deconvolute the signals, allowing for the precise localization and quantification of each biomarker.
