What Are the Challenges Associated with Deep Tissue Imaging?
Despite its advantages, deep tissue imaging faces several challenges: - Photobleaching and Phototoxicity: Extended imaging times can lead to photobleaching of fluorophores and potential damage to live tissues. - Resolution: Achieving high resolution at great depths remains a technical hurdle. - Data Handling: The large datasets generated require significant computational resources for storage and analysis. - Tissue Preparation: Effective tissue clearing and labeling can be time-consuming and technically demanding.
