What are the Common Techniques Used in Deep Tissue Imaging?
Several techniques are widely employed for deep tissue imaging:
1. Two-Photon Microscopy: This technique uses two photons of lower energy to excite fluorophores. It allows for deeper tissue penetration and reduced phototoxicity. 2. Clearing Methods: Techniques like CLARITY, Scale, and iDISCO make tissues transparent, allowing for deeper light penetration and clearer imaging. 3. Light Sheet Microscopy: This method illuminates a thin section of the sample, reducing photodamage and enabling rapid imaging of large volumes. 4. Optical Coherence Tomography (OCT): OCT uses light waves to capture micrometer-resolution images from within optical scattering media like biological tissues. 5. Magnetic Resonance Imaging (MRI): Though typically associated with clinical imaging, high-resolution MRI can also be used for deep tissue histological studies.
