What are the Challenges Associated with Alkaline Denaturation?
While alkaline denaturation is a valuable tool in histology, it comes with challenges:
1. Over-Denaturation: Excessive exposure to alkaline conditions can lead to over-denaturation, making the tissue sample unusable for further analysis. 2. pH Control: Maintaining precise pH levels is crucial to avoid damaging the tissue or affecting the specificity of the stains. 3. Protein Loss: Prolonged exposure to alkaline solutions can lead to the loss of proteins, which may be critical for certain diagnostic purposes.
