The use of cholera toxin B in histological studies offers several benefits:
High specificity: CTB binds specifically to GM1 gangliosides, ensuring precise labeling. Minimal toxicity: CTB alone is non-toxic, making it safer for use in live tissue studies. Retrograde and anterograde tracing: CTB can be used to trace both the origins and destinations of neuronal pathways. Versatility: It can be conjugated with various markers to suit different experimental needs.
