What are the Basic Steps in Histological Preparation?
The preparation of histological slides involves several key steps:
Fixation: Preserving the tissue to prevent decay. Embedding: Encasing the tissue in a solid medium, usually paraffin wax, to allow thin sections to be cut. Sectioning: Cutting thin slices of tissue using a microtome. Staining: Applying dyes to highlight different structures within the tissue. Mounting: Placing the tissue section on a glass slide for examination under the microscope.