Sample Preparation: Tissue samples are first prepared, often involving homogenization, extraction, and filtration to isolate the molecules of interest. Application: The prepared sample is then applied to the chromatography medium, which could be a column, plate, or other substrate. Separation: As the sample moves through the medium, the different components separate based on their unique physical and chemical properties. Detection: The separated components are detected and often quantified using various detection methods such as UV-VIS spectrophotometry, mass spectrometry, or fluorescence.
