What are the Applications of In Vitro Transcription in Histology?
In vitro transcription has several applications in histology, including:
- Generating RNA Probes: For RNA in situ hybridization, RNA probes are used to detect specific RNA sequences within tissue sections, providing insights into gene expression and localization. - Studying Gene Expression: Transcribed RNA can be introduced into cells to study the effects of overexpression or knockdown of specific genes. - Creating RNA Standards: For quantitative PCR and other analytical techniques, RNA standards synthesized in vitro serve as controls.
