What are the Applications of Freezing in Histology?
Freezing has several important applications: - Intraoperative Consultation: Surgeons often rely on frozen sections to make immediate decisions during surgery, such as determining the margins of a tumor. - Enzyme Histochemistry: Freezing is essential for studying enzyme activities, as it preserves the enzymatic integrity. - Molecular Studies: Frozen tissues are often used in molecular biology for techniques such as PCR and Western blotting, where preservation of nucleic acids and proteins is critical.
