The freezing process typically involves the following steps: - Tissue Collection: A small piece of tissue is collected using a scalpel or biopsy needle. - Embedding: The tissue is embedded in a medium such as OCT compound to support the tissue structure during sectioning. - Freezing: The tissue is rapidly frozen using liquid nitrogen, isopentane, or a cryostat. Liquid nitrogen is effective due to its extremely low temperature, while isopentane is often used to avoid tissue cracking. - Sectioning: The frozen tissue block is sectioned using a cryostat, producing thin slices that can be mounted on slides for staining and examination.
