- Preservation of Sample Integrity: Without the introduction of external agents, the tissue remains in its natural state, which is crucial for accurate analysis. - Reduced Preparation Time: Eliminating the need for staining or labeling significantly reduces the time required to prepare samples. - Cost-Effectiveness: The absence of reagents lowers the overall cost of the procedure. - Live Imaging: Techniques like multiphoton microscopy and OCT allow for the observation of live tissues, which is invaluable for dynamic studies.