Recent advancements have improved the capabilities of RT-PCR in histology:
- Real-Time RT-PCR: Allows for the real-time monitoring of the amplification process, providing immediate data on gene expression levels. - Digital PCR: Offers higher sensitivity and precision by partitioning the sample into thousands of individual reactions. - Multiplex RT-PCR: Enables the simultaneous detection of multiple genes in a single reaction, saving time and resources.