The process of RT-PCR in histology involves several key steps:
1. RNA Extraction: RNA is extracted from tissue samples using specialized kits that ensure high purity and integrity. 2. Reverse Transcription: The extracted RNA is converted into cDNA using reverse transcriptase enzyme. 3. PCR Amplification: The cDNA is then amplified using specific primers that target the genes of interest. 4. Detection and Quantification: The amplified products are detected and quantified, often using fluorescent dyes that provide real-time data on gene expression levels.
