What are Some Techniques to Address Cross Reactivity?
- Double-Labeling: Using two different antibodies that target different epitopes of the same antigen can help confirm specificity. - Sequential Staining: Staining with one antibody, stripping the section, and then staining with a second antibody can help identify cross-reactivity issues. - Advanced Imaging: Techniques like confocal microscopy can provide more precise localization of antigens, helping to distinguish specific from non-specific staining.
