Staining is essential for highlighting specific tissue components. Here are a few specialized techniques:
Hematoxylin and Eosin (H&E): Hematoxylin stains cell nuclei blue, while Eosin stains cytoplasm and extracellular matrix pink. Masson's Trichrome: Differentiates between muscle (red), collagen (blue or green), and cell nuclei (black). Periodic Acid-Schiff (PAS): Stains carbohydrates and glycogen magenta. Immunohistochemistry (IHC): Uses antibodies to detect specific proteins, often visualized with chromogenic or fluorescent markers.
