Staining is essential for revealing the intricate details of tissues under a microscope. Common techniques include:
Hematoxylin and Eosin (H&E): The most widely used stain that highlights nuclei and cytoplasmic components. Immunohistochemistry (IHC): Uses antibodies to detect specific proteins within tissues, useful for identifying disease markers. Masson's Trichrome: Differentiates between muscle, collagen, and fibrin, useful in studying fibrosis. Periodic Acid-Schiff (PAS): Highlights carbohydrates and mucopolysaccharides, often used in kidney and liver studies.