What are Common Types of Genetically Encoded Reporters?
Several types of genetically encoded reporters are frequently used in histology:
1. Fluorescent Proteins: These include GFP, RFP (Red Fluorescent Protein), and YFP (Yellow Fluorescent Protein). They are widely used because they provide a direct, visible signal that can be observed using fluorescence microscopy. 2. Luciferase: This enzyme catalyzes a reaction that produces light, which can be detected using a luminometer. It is commonly used in bioluminescence imaging. 3. β-galactosidase: Often used in X-gal staining assays, this enzyme produces a blue color in the presence of its substrate, allowing for easy visualization.
