Autolysis: Delay in fixation can lead to enzymatic degradation of tissues. Over-fixation: Prolonged fixation can cause hardening and shrinkage of tissues, affecting morphology. Improper Orientation: Incorrect orientation during embedding can result in non-representative sections. Inadequate Dehydration: Insufficient dehydration can lead to poor infiltration and section quality. Contamination: Use of non-sterile instruments or improper handling can introduce contaminants.
