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What Are Common Chromogenic Substrates?
Several chromogenic substrates are widely used in histology. Some of the most popular ones include:
3,3'-Diaminobenzidine (DAB)
– produces a brown color and is often used with HRP.
Fast Red
– used with AP to produce a red color.
4-Chloro-1-Naphthol (4-CN)
– results in a blue color when used with HRP.
These substrates are chosen based on the desired contrast and compatibility with other stains in multi-label experiments.
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