Recent advances are enhancing the accuracy and capabilities of visual localization:
Confocal Microscopy: Provides optical sectioning and 3D reconstruction of tissues, improving resolution and localization accuracy. Multiplex Staining: Allows for the simultaneous detection of multiple targets within a single tissue section, offering a more comprehensive view of cellular interactions. Super-Resolution Microscopy: Breaks the diffraction limit of light microscopy, enabling the visualization of structures at the nanometer scale.