Optimal Fixation
Ensure that tissue samples are fixed promptly and adequately. The choice of fixative, fixation time, and penetration efficiency are critical factors. For example, using a 10% neutral buffered formalin and fixing for 12-24 hours is commonly recommended.
Proper Storage
Store fixed samples in appropriate conditions, such as refrigeration (4°C) for short-term storage or freezing (-80°C) for long-term storage. Avoid repeated freeze-thaw cycles to prevent ice crystal formation and tissue damage.
Minimize Mechanical Stress
Handle tissue samples gently to avoid mechanical damage. Use sharp instruments and avoid excessive force during cutting and sectioning. Employ proper embedding techniques to support tissue integrity during microtomy.
Controlled Chemical Exposure
Follow standardized protocols for chemical exposure, including fixation, dehydration, clearing, embedding, and staining. Avoid prolonged exposure to harsh chemicals and ensure thorough washing steps to remove residual reagents.
Ensure Sterility
Maintain a sterile environment during sample collection, processing, and storage. Use sterile instruments and containers, and implement good laboratory practices to prevent microbial contamination.
