There are several reasons why folding might occur in histological sections. These include:
Improper embedding of the tissue in the paraffin block, leading to uneven surfaces. Sectioning technique errors, such as incorrect angle or pressure applied during microtomy. Variations in tissue consistency, where tougher or more fibrous areas fold more easily. Mounting errors, such as improper placement on the slide or inadequate stretching of the section in the water bath.
