What are the techniques to study mTORC2 in Histology?

Several techniques can be employed to study mTORC2 in the context of Histology. Immunohistochemistry (IHC) and immunofluorescence are commonly used to localize and visualize mTORC2 components in tissue sections. Western blotting and RT-PCR can be used to quantify the expression levels of mTORC2 and its downstream targets. Additionally, genetic models, such as knockout mice, can provide insights into the functional roles of mTORC2 in vivo.