What is the Role of pH and Temperature in Fixation?
The pH and temperature of the fixative can significantly impact the fixation process:
pH: Most fixatives work optimally at a pH range of 7.2-7.4. Deviations from this range can affect the protein structure and tissue morphology. Temperature: Fixation is usually carried out at room temperature (20-25°C). Higher temperatures can accelerate fixation but may also increase the risk of tissue artifacts.
