The procedure for Prussian Blue staining typically involves the following steps:
1. Fixation: Tissue samples are fixed using an appropriate fixative, such as formalin. 2. Sectioning: The fixed tissue is embedded in paraffin and sectioned into thin slices. 3. Deparaffinization and Hydration: The sections are deparaffinized and hydrated through a series of graded alcohols. 4. Staining: The sections are treated with a mixture of potassium ferrocyanide and hydrochloric acid. 5. Counterstaining: A counterstain, such as nuclear fast red, may be applied to provide contrast. 6. Dehydration and Mounting: The sections are dehydrated, cleared, and mounted for microscopic examination.
