Despite its advantages, the MTT assay has some limitations:
Interference: Certain compounds and cell types can interfere with the assay, leading to inaccurate results. Non-Specificity: The assay measures overall metabolic activity, which may not always correlate with cell viability. Cell Density: High cell densities can lead to the overproduction of formazan, affecting the accuracy of the results. Solubility Issues: The solubilization step can sometimes be problematic, especially with certain cell types.
