Minimizing preparation artifacts involves careful attention to each step of the histological process. Here are some tips:
Proper Fixation: Use the appropriate fixative and fixation time for the tissue type. Ensure that tissues are adequately penetrated by the fixative. Controlled Dehydration: Gradually dehydrate tissues using a graded series of alcohols to avoid rapid shrinkage or hardening. Optimal Embedding: Ensure complete infiltration of embedding medium and avoid overheating the medium. Careful Sectioning: Use a sharp microtome blade and adjust the cutting speed and angle to minimize sectioning artifacts. Consistent Staining: Follow standardized staining protocols and use fresh reagents to ensure even and accurate staining.
