To minimize fixation artifacts, several strategies can be employed:
- Optimize Fixative Choice: Select a fixative that is suitable for the tissue type and the intended downstream analyses. For example, glutaraldehyde is excellent for preserving ultrastructure, while formaldehyde is widely used for general histological studies. - Control Fixation Time: Adhere to recommended fixation times to avoid under or over-fixation. Typically, formalin fixation is performed for 24-48 hours. - Ensure Adequate Penetration: Slice tissues into thin sections, ideally 3-5mm thick, to facilitate uniform fixative penetration. - Maintain Optimal Conditions: Keep the fixative at the appropriate pH and temperature to ensure effective fixation without inducing artifacts.
