Proper use of a vacuum autoclave involves several steps: 1. Loading: Ensure items are loaded in a way that allows steam to circulate freely. 2. Cycle Selection: Choose the appropriate cycle based on the materials being sterilized. 3. Monitoring: Use biological indicators and chemical indicators to verify that sterilization parameters are met. 4. Maintenance: Perform regular maintenance as per the manufacturer's guidelines to ensure the device's longevity and reliability.