The procedure for Warthin-Starry staining involves several steps:
Fixation of the tissue sample in formalin. Dehydration through a series of alcohols and clearing with xylene. Embedding the tissue in paraffin and sectioning it thinly. Rehydration and application of the silver nitrate solution. Reduction of silver in the presence of the bacteria using a developer solution. Rinsing, dehydrating, and mounting the stained sections for microscopic examination.
