Several techniques are employed to study tissue specific expression. These include:
1. Immunohistochemistry (IHC): This technique uses antibodies to detect specific proteins in tissue sections. It allows for the visualization of the spatial distribution of proteins within tissues.
2. In Situ Hybridization (ISH): ISH is used to localize specific nucleic acid sequences within tissue sections. This technique can identify where and when specific genes are expressed.
3. RNA Sequencing (RNA-seq): RNA-seq is a powerful method to quantify and compare gene expression across different tissues. It provides a comprehensive view of the transcriptome.
4. Microarray Analysis: Microarrays can measure the expression levels of thousands of genes simultaneously, providing a broad picture of tissue specific expression patterns.
