The process of SDH staining involves several steps: 1. Preparation of Tissue Sections: Fresh frozen sections of tissue are typically used. The tissue is cut into thin slices using a cryostat. 2. Incubation with SDH Substrate: The sections are incubated in a solution containing sodium succinate and an electron acceptor like nitro blue tetrazolium (NBT). SDH activity reduces NBT to a blue formazan product, indicating active enzyme sites. 3. Washing and Mounting: After incubation, the sections are washed to remove excess substrate and then mounted on slides for microscopic examination.
