Histological examination of the RPE involves several techniques: - Light Microscopy: Standard staining methods like hematoxylin and eosin (H&E) to observe cell structure. - Electron Microscopy: Provides detailed images of RPE ultrastructure, including microvilli and intercellular junctions. - Immunohistochemistry: Used to detect specific proteins and markers associated with RPE function and pathology. - Fluorescence Microscopy: Helps in visualizing the distribution of pigments and other fluorescent molecules within RPE cells.
