The diagnosis of the loss of rete ridges is primarily made through histological examination. A biopsy of the affected skin is typically prepared and stained using techniques like Hematoxylin and Eosin (H&E) staining. Under a microscope, the histologist can observe the flattening or absence of rete ridges. Immunohistochemical stains may also be used to assess the presence of specific markers that can provide additional information about the underlying cause.
