Sample Collection: During surgery, a sample of the tissue is collected and sent to the pathology lab. Freezing: The tissue sample is quickly frozen using a cryostat, which is a specialized instrument that maintains very low temperatures. This preserves the tissue structure and makes it easier to cut. Sectioning: The frozen tissue is then cut into very thin sections using a microtome within the cryostat. Staining: The thin sections are stained with appropriate dyes to highlight different cellular components. Microscopic Examination: A pathologist examines the stained sections under a microscope and provides a diagnosis.
