The EVG staining procedure involves several steps: 1. Fixation: Tissue samples are fixed, commonly using formalin. 2. Deparaffinization and Hydration: Paraffin-embedded tissue sections are deparaffinized and rehydrated through a series of alcohol solutions to water. 3. Staining with Verhoeff’s Hematoxylin: The sections are stained with Verhoeff’s hematoxylin, which binds to elastic fibers. 4. Differentiation: The tissue is then differentiated in ferric chloride, which removes excess stain. 5. Counterstaining with Van Gieson: Finally, the sections are stained with Van Gieson’s solution to visualize collagen and other connective tissue components. 6. Dehydration and Mounting: The stained sections are dehydrated, cleared, and mounted for microscopic examination.
