How is Succinate Dehydrogenase Activity Detected in Histological Studies?
SDH activity can be detected using histochemical staining techniques. One common method involves incubating tissue sections with a solution containing succinate and a dye like nitro blue tetrazolium (NBT). The SDH enzyme catalyzes the oxidation of succinate, which in turn reduces NBT, resulting in a blue precipitate that indicates areas of high SDH activity.
