Preparation: Sterilize all equipment and materials. Warm the growth medium and trypsin solution to the appropriate temperature. Detachment: Remove the old medium, rinse cells with PBS, and add trypsin to detach adherent cells. Incubate for a few minutes until cells round up and detach. Neutralization: Add fresh growth medium to neutralize the trypsin and gently pipette to create a single-cell suspension. Transfer: Transfer a portion of the cell suspension into a new culture vessel containing fresh growth medium. Incubation: Place the new culture vessel in the incubator and monitor cell growth regularly.
